[Development of an LC-MS/MS Method for Determining Residual Isoxaflutole and Metabolite B in Livestock Products].

Authors

Hiroyuki Kikuchi, Takatoshi Sakai, Tomoko Okura, Satoru Nemoto, Hiroshi Akiyama, Takaaki Taguchi, Tomoaki Tsutsumi

Published in

Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan. Volume 67. Issue 5. Pages 205-213.

Abstract

A reliable LC-MS/MS method was developed for determining residual isoxaflutole and its primary metabolite, metabolite B (isoxaflutole diketonitrile), in livestock products. Isoxaflutole is an unstable compound that rapidly degrades to its metabolites. To prevent the conversion of isoxaflutole to its metabolites during sample preparation, a mixture of 4 mol/L HCl and ethanol (1 : 1, v/v) was added to the sample in an amount equivalent to half of the sample weight and mixed. Analytes were extracted with acetonitrile in the presence of n-hexane, followed by partitioning with a mixture of ethyl acetate and n-hexane (3 : 7, v/v) and a sodium chloride solution. The organic layer was cleaned up using an Oasis HLB cartridge and then analyzed by LC-MS/MS. Validation was performed through recovery tests using five livestock products. Samples were spiked with isoxaflutole and metabolite B at 0.01 mg/kg and at the maximum residue limits established for each food. The validation results for the analytes showed excellent recoveries (80-94%) and high precision (<10%).

PMID:
42968336
Bibliographic data and abstract were imported from PubMed on 02 Nov 2026.

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