3-Oxo-C10-HSL accumulation and functional responses of Pseudomonas aeruginosa in anoxic bioelectrochemical systems for wastewater treatment and valorization: effects of C/N ratio and nitrate availability.

Authors

Nahid Movahedi, Reza Panahi, Farshad Yazdani, Hamid Moghimi

Published in

Biodegradation. Volume 37. Issue 6. Nov 02, 2026. Epub Nov 02, 2026.

Abstract

Understanding how environmental conditions influence microbial functions is essential for optimizing biological wastewater treatment and bioelectrochemical systems. This study investigated the effects of carbon-to-nitrogen (C/N) ratio and nitrate availability on 3-oxo-C10-HSL accumulation and the functional responses of Pseudomonas aeruginosa 9027 under anoxic conditions using microbial fuel cells (MFCs). Initial C/N ratios of 1, 10, and 30 were established using sodium acetate and KNO₃. After 240 h of operation, 3-oxo-C10-HSL concentrations were 18.9 ± 16.4, 91.1 ± 59.5, and 202.3 ± 51.3 nM at C/N ratios of 1, 10, and 30, respectively, while residual nitrate concentrations were 7.5 ± 1.5, 1.5 ± 0.4, and 0.8 ± 0.7 mM. 3-oxo-C10-HSL was not detected in the limited aerobic experiments conducted for comparison. 3-oxo-C10-HSL accumulation was associated with nitrate depletion and was greater at higher initial C/N ratios following nitrate depletion. COD and nitrate removal exceeded 89% and 91%, respectively. PYO accumulation was higher at lower C/N ratios, whereas biofilm thickness increased from approximately 25 to 40 and 60 µm at C/N ratios of 1, 10, and 30, respectively. The C/N ratio also affected MFC electrochemical performance. Overall, the results indicate that C/N ratio and nitrate availability were associated with 3-oxo-C10-HSL accumulation and multiple functional responses of P. aeruginosa 9027 under anoxic conditions. These findings highlight C/N ratio and nitrate availability as important environmental factors that may be considered for regulating microbial processes in wastewater-treatment and bioelectrochemical systems.

PMID:
42970021
Bibliographic data and abstract were imported from PubMed on 02 Nov 2026.

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