miR-8485 by Targeting ITGAM Expression Alleviates Cognitive Impairment Induced by Ischemic Stroke.

Authors

Wei Shen, Lei Gao, Guoqing Liu, Zicheng Li, Jinhai Li

Published in

Journal of geriatric psychiatry and neurology. Pages 8919887261503009. Nov 03, 2026. Epub Nov 03, 2026.

Abstract

ObjectivePost-stroke cognitive impairment (PSCI) is a major complication of ischemic stroke (IS). This study aims to explore the role of miR-8485 in PSCI and its underlying molecular mechanism.Methods84 PSCI patients and 90 post-stroke cognitively normal (PSCN) patients were included. Serum miR-8485 and ITGAM expression were examined via reverse transcription quantitative polymerase chain reaction. The diagnostic efficacy of miR-8485 was evaluated by receiver operating characteristic (ROC) curve. Risk factors of PSCI were determined via logistic regression analysis. An in vitro PSCI cell model was constructed through oxygen-glucose deprivation/reoxygenation (OGD/R). Cell viability, apoptosis and inflammatory factor levels were determined by Cell Counting Kit-8 assay, flow cytometry and enzyme-linked immunosorbent assay. The oxidative stress was evaluated by measuring superoxide dismutase (SOD), malondialdehyde (MDA) and catalase (CAT) levels. The interaction between miR-8485 and ITGAM was verified by dual-luciferase reporter assay.ResultsmiR-8485 was downregulated in PSCI patients, and ROC analysis showed that it could differentiate PSCI patients from PSCN patients. Downregulated miR-8485 expression was associated with PSCI disease severity. In OGD/R-treated human microglial cells (HMC3), upregulation of miR-8485 increased cell viability, suppressed inflammatory responses and oxidative stress, and inhibited apoptosis. ITGAM was the target of miR-8485. Overexpression of ITGAM partially reversed the protective effect of miR-8485 upregulation in OGD/R-induced HMC3 cells.ConclusionmiR-8485 alleviates OGD/R-induced inflammatoty responses and oxidative stress via targeted inhibition of ITGAM expression. The miR-8485/ITGAM axis may serve as a key regulatory pathway and a potential therapeutic candidate for further investigation in PSCI.

PMID:
42975094
Bibliographic data and abstract were imported from PubMed on 03 Nov 2026.

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