A Hydrogen Peroxide-Responsive Fluorescent Probe for Fluorescence and Lifetime Imaging of Oxidative Stress in Diabetes.

Authors

Qian Wang, Ying Bi, Zheng-Guo Yin, Yun-Tao Li, Xiao-Ming Mao

Published in

Luminescence : the journal of biological and chemical luminescence. Volume 41. Issue 11.

Abstract

Hydrogen peroxide (H₂O₂), as one of the key reactive oxygen species (ROS), plays dual roles as a signaling molecule and a mediator of oxidative damage in biological systems. Abnormal accumulation of H₂O₂ is closely associated with metabolic disorders, particularly diabetes, in which excessive oxidative stress can contribute to cellular dysfunction and disease progression. Therefore, effective strategies for monitoring dynamic changes in intracellular H₂O₂ are important for understanding redox-related pathological processes. Herein, we developed a H₂O₂-responsive fluorescent probe (HOP) for real-time visualization of intracellular oxidative stress. HOP exhibited good biocompatibility and enabled fluorescence imaging of both exogenous and endogenous H₂O₂ in living 3T3 cells. In addition, fluorescence lifetime imaging microscopy (FLIM) revealed distinct changes in fluorescence lifetime upon H₂O₂ activation. By combining fluorescence intensity-based imaging with lifetime imaging, H₂O₂-associated oxidative stress in diabetic cells could be visualized from complementary optical readouts. This work provides a molecular imaging tool for investigating dynamic changes in H₂O₂ and offers insights into oxidative stress-associated metabolic disorders.

PMID:
43003403
Bibliographic data and abstract were imported from PubMed on 10 Nov 2026.

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