Authors
Qi Jiao, Zhisong Wang, Qiuqian Gu, Yijing Tao, Ping Zou, Qianwen Yao, Juan Wang
Published in
Mikrochimica acta. Volume 193. Issue 12. Nov 10, 2026. Epub Nov 10, 2026.
Abstract
A magnetic separation-assisted competitive fluorescent aptasensor based on CdTe quantum dots (CdTe QDs) and Ag⁺ for the sensitive and selective detection of heart-type fatty acid binding protein (FABP3) in clinical serum samples has been developed. CdTe QDs were first quenched by Ag⁺ to form a CdTe QDs-Ag⁺ complex in the off state. The aptamer was immobilized on magnetic beads and hybridized with poly-T to form an MB-Apt/poly-T duplex. In the presence of FABP3, the aptamer preferentially bound to FABP3, leading to the release of poly-T from the magnetic beads. After magnetic separation, the released poly-T in the supernatant bound to Ag⁺ and competed with CdTe QDs for Ag⁺, thereby removing Ag⁺ from the CdTe QDs surface and restoring the fluorescence of CdTe QDs. In the absence of FABP3, poly-T remained on the magnetic beads, and the supernatant showed no fluorescence recovery. Under optimized conditions, the sensor exhibited a linear response to FABP3 in the range 1-20 ng/mL, with a detection limit of 0.087 ng/mL. The method also showed excellent selectivity toward FABP3 against other interfering substances. In clinical serum samples, the sensor successfully distinguished healthy individuals from patients with myocardial injury. The results showed good agreement with a commercial ELISA kit (R² = 0.98), with a mean bias of -0.16 ng/mL and 95% limits of agreement from - 1.70 to + 1.39 ng/mL. Recoveries were 97.2-104.3% in 20% serum.
PMID:
43007466
Bibliographic data and abstract were imported from PubMed on 11 Nov 2026.
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