Structural Characterization and In Vitro Biological Activity of an Extracellular Polysaccharide from Lactobacillus paracasei SMR-0128.

Authors

Jia Song, Xikang Xue, Shangzu Gu, Shuo Wang, Chenrui Zhen, Bingning Gao, Chenyu Yang, Min Wang, Yu Zheng

Published in

Journal of agricultural and food chemistry. Volume 74. Issue 44. Pages 34739-34753. Nov 11, 2026.

Abstract

Lactobacillus paracasei produces bioactive exopolysaccharides, but their structures remain poorly defined. Here, an exopolysaccharide fraction, LPP (12.3 kDa), was isolated from L. paracasei SMR-0128. LPP was mainly composed of rhamnose and mannose, with glucose, glucuronic acid, N-acetylgalactosamine, arabinose, glucosamine, galactose, and xylose. Methylation and diagnostic NMR data indicated abundant terminal and substituted Rha/Man residues, possible branching sites, and acidic and acetyl/N-acetyl-related features; however, the complete repeating unit could not be resolved. LPP was therefore characterized as a Rha- and Man-rich branched acidic heteropolysaccharide containing multiple terminal and substituted residues, while its complete repeating-unit architecture remained unresolved. Functionally, LPP enhanced phagocytosis, nitric oxide production, and cytokine secretion in RAW264.7 macrophages under basal conditions, while modulating NF-κB-related signaling and reducing selected inflammatory mediators in LPS-stimulated cells. These findings support further investigation of LPP as a probiotic-derived immunostimulatory polysaccharide.

PMID:
43012681
Bibliographic data and abstract were imported from PubMed on 11 Nov 2026.

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