Authors
Jiashu Wang, Guohua Wu, Qijun Du, Di Wu, Qinrui Lu, Ao Xie, Wenqi Hu, Chenwei Sun, Zipeng Yao, Haijie Hu, Bangchuan Hu, Shuqi Wang
Published in
Lab on a chip. Nov 12, 2026. Epub Nov 12, 2026.
Abstract
Many three-dimensional (3D) skin equivalents are established separately from microfluidic devices and are not readily compatible with downstream organ integration. We developed a microfluidic epidermal-dermal skin model using a composite hydrogel comprising a decellularized skin matrix (DSM) and gelatin methacryloyl (GelMA), termed G-DSM. Combined with interstitial perfusion and air-liquid interface (ALI) culture, the platform supported the establishment of a day-7 construct containing epidermal and dermal compartments. The construct expressed keratin 14 (K14), keratin 10 (K10), claudin-1, and fibronectin and showed lower apparent permeability to BSA and benzoic acid than the dermal-only construct, together with a water vapor transmission rate close to that of native skin. Functionally, the model exhibited concentration-dependent PICP and fibronectin responses to VC and retinol, with an intermediate-concentration combination producing greater responses than the corresponding single-agent treatments. The model also distinguished concentration-dependent local cytotoxicity among five selected chemicals and generated a study-specific exploratory ranking. Coupling the skin construct to a downstream liver compartment showed that the transdermal route reduced liver-inlet APAP exposure, while approximately AUC-matched comparisons indicated that the route-associated differences in ROS and viability were largely exposure-dependent.
PMID:
43016207
Bibliographic data and abstract were imported from PubMed on 12 Nov 2026.
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