Authors
Tina Batista Napotnik, Anja Blažič, Vid Jan, Klara Bulc Rozman, Rok Šmerc, Lea Rems
Published in
Scientific reports. Volume 16. Pages 34459. Epub Jul 28, 2026.
Abstract
In recent years, irreversible electroporation has been increasingly used to treat cardiac arrhythmias, an approach termed pulsed field ablation. The mechanisms of how electroporation affects the generation and propagation of action potentials (AP) in the myocardium (and other excitable tissues) remain to be elucidated. In our previous in vitro studies using genetically engineered S-HEK cells as a simple model of excitable cells we identified two unexpected phenomena that remain unexplained: (1) multiple APs triggered by a single electric pulse, and (2) a pulse-induced complex (bi- or multiphasic) calcium response not observed in their non-excitable counterparts NS-HEK cells. In this study, we employed fluorescence microscopy for optical monitoring of APs and intracellular calcium, pharmacological interventions targeting specific ion channels, and molecular biology techniques to characterize the expression of selected ion channels in S-HEK and NS-HEK cells. Our findings show that (1) multiple APs result primarily from sustained AP propagation along the cell monolayer through gap junctions, and (2) the complex calcium response in S-HEK cells reflects synchronized calcium influx during these propagating APs, with extracellular calcium being primary source rather than internal store release.
The online version contains supplementary material available at https://doi.org/10.1038/s41598-026-63712-8.
PMID:
43019146
Bibliographic data and abstract were imported from PubMed on 13 Nov 2026.
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