Authors
Yang Su, Maiya Callender, Maor Bar-Peled, Eric T Harvill, Thomas Krunkosky
Published in
Scientific reports. Volume 16. Pages 37492. Epub Jul 31, 2026.
Abstract
Nasal epithelia are amongst the first lines of defense against airway infections. Development of a robust ex vivo nasal epithelia model will enable further mechanistic study of pathogen-upper airway interaction. While the current human nasal epithelia models are limited by their availability and ethical constraints, there is a lack of a robust mouse nasal epithelia model. Here, we developed a functional mouse primary nasal epithelia (MNE) model in an air-liquid interface (ALI). We described an intriguing property of MNE to lose confluence and trans-epithelial electrical resistance (TEER) when initially exposed to ALI conditions, which explains the prior challenges in the development of MNE. Our novel procedure employed two cycles of growth and differentiation to obtain a confluent layer of MNE in ALI. Furthermore, we established MNE as an infectious disease model using Bordetella bronchiseptica, a broad-host spectrum airway pathogen of various animals, including mice. B. bronchiseptica efficiently attached to and colonized the apical face of the MNE, followed by proliferation and disruption of the MNE barrier. Immunostaining indicated that B. bronchiseptica bound cilia of MNE, as they do in vivo in mice. Our results highlight the primary MNE as a powerful tool for studying the interaction between respiratory bacteria and airway epithelia.
The online version contains supplementary material available at https://doi.org/10.1038/s41598-026-63086-x.
PMID:
43019159
Bibliographic data and abstract were imported from PubMed on 13 Nov 2026.
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