Protocol for filament-induced transient middle cerebral artery occlusion with laser speckle imaging and outcome assessment in mice.

Authors

Junchang Xie, Yugang Wang, Li Jiang

Published in

STAR protocols. Volume 7. Issue 4. Pages 104962. Nov 13, 2026. Epub Nov 13, 2026.

Abstract

The transient middle cerebral artery occlusion (tMCAO) model in mice is important for investigating endogenous protective mechanisms and potential therapeutic strategies for ischemic stroke. Here, we present a protocol for verifying successful occlusion and reperfusion using filament-induced tMCAO, with real-time monitoring of cerebral blood flow by laser speckle contrast imaging (LSCI). We also describe steps for neurological testing, 2,3,5-triphenyltetrazolium chloride (TTC) staining, and representative histological analyses for assessing ischemic brain injury and model quality. For complete details on the use and execution of this protocol, please refer to Jiang et al.¹.

PMID:
43024512
Bibliographic data and abstract were imported from PubMed on 14 Nov 2026.

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