10x TAE buffer (10x Tris-acetate-EDTA)

Summary

TAE buffer is typically used for agarose DNA electrophoresis.

Materials

To prepare 1L of 10x solution, you need:

• 48.5 g Tris
• 11.4 mL glacial acetic acid
• 20 mL 0.5M EDTA (pH 8.0)

Procedure

1. Dissolve Tris in about 800 mL of deionized water.
2. Add acetic acid and EDTA.
3. Add deionized water to 1L.
4. Store at room temperature.

Dilute stock solution 10:1 to make a 1x working solution.

1x buffer will contain 40 mM Tris, 20 mM acetic acid and 1 mM EDTA.

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Comments

• 

  Alen Piljić Friday, 10 January 2014 - 00:10 UTC

  OK, thanks for sharing. The link is here:
  http://www.lifescience.net/protocols/53/sodium-borate-buffer-for-rapid-dna-electrophoresis/

• Bradley Stevenson Thursday, 09 January 2014 - 23:42 UTC

  Sodium borate is an alternative buffer for DNA electrophoresis that allows faster run times and uses cheaper reagents. The recipe can be found on this website.