10x TBE buffer (10x Tris-borate-EDTA)

Summary

TBE buffer is typically used for agarose DNA electrophoresis.

Materials

To prepare 1L of 10x solution, you need:

• 108 g Tris base
• 55 g boric acid
• 40 mL 0.5M EDTA (pH 8.0)

Procedure

1. Dissolve Tris in about 800 mL of deionized water.
2. Add boric acid and EDTA.
3. Add deionized water to 1L.
4. Store at room temperature.

Dilute stock solution 10:1 to make a 1x working solution.

0.5x solution can also be used.

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Comments

• farhat ashraf Tuesday, 05 January 2016 - 20:52 UTC

  please tell me how to prepare agarose gel for dna electrophoresis

• farhat ashraf Tuesday, 05 January 2016 - 20:37 UTC

  thanks for help. it was very useful for my assignment