Microglial motility

Authors

Unknown author

Procedure

Directed motility of microglia under different conditions of stimulation was evaluated
according to a previously described protocol (Czapiga and Colton, 1999) with few
modifications: 25 μl of adenosine diphosphate (ADP; 10 μM) or control vehicle (PBS)
were placed around the perimeter of unstimulated, acute or chronic OHSCs and the
slices were then incubated for 4 hrs, fixed with PFA and stained with CD68 for
microglia visualization. The concentration of ADP (10 μM) used was chosen on the
basis of the previously reported effectiveness of ADP as a chemoattractant for microglia
(De Simone et al., 2010).

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