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Protocols

Protocols module of the Life Science Network enables sharing of detailed experimental protocols, laboratory recipes and data analysis procedures.

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External protocol30 Apr 2014

Tensin-PTBc expression and purification

by Robert Liddington and Andrey Bobkov
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External protocol30 Apr 2014

Full protein sequence mapping using mass spectrometry

by Donald Hunt and Erin Jeffery
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External protocol10 Apr 2014

Use of an anaerobic chamber environment for the assay of endogenous cellular protein-tyrosine phosphatase activities

by Li Zhu, Barry Goldstein
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External protocol30 Apr 2014

Optimized immunofluorescence staining protocol to detect the nucleoporin Nup98 in different subcellular compartments

by Mohamed Kodiha, Rehan Umar, and Ursula Stochaj
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External protocol30 Apr 2014

Desktop scanner replaces spectrophotometer for low cost and rapid ELISA technique

by Pradip Nahar & Shahila Parween
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External protocol30 Apr 2014

Injection of Retrograde Beads into the Nucleus Accumbens (NAc) and Medial Prefronral Cortex (mPFC) to Isolate Projection-Specific Neurons in the Ventral Tegmental Area (VTA)

by Jessica Walsh*, Allyson Friedman*, Dipesh Chaudhury*, Barbara Juarez, Stacy Ku, and Ming-Hu Han
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External protocol30 Apr 2014

Production of polyclonal antibodies against protein antigens purified by electroelution from SDS-polyacrylamide gel

by Massimo Plumari, Cinzia Gellera, and Franco Taroni
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External protocol03 May 2014

Prediction of allergenic proteins and mapping of IgE epitopes in allergens

by Sudipto Saha and Gajendra Raghava
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External protocol03 May 2014

Investigating histone deubiquitination: Xenopus manipulations

by Hengbin Wang and Chenbei Chang
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External protocol30 Apr 2014

Non-invasive measurement of real-time oxygen flux in plant systems with a self-referencing optrode

by Yinglang Wan, Eric McLamore, Lusheng Fan, Huaiqing Hao, D. Marshall Porterfield, Zengkai Zhang, Wenjun Wang, Yue (Jeff) Xu, and Jinxing Lin
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