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Protocols

Protocols module of the Life Science Network enables sharing of detailed experimental protocols, laboratory recipes and data analysis procedures.

You can upload a protocol here

External protocol30 Apr 2014

Analysis of D-loop stability in DMC1 and RAD51 driven strand invasion reactions of homologous recombination

by Dmitry Bugreev, Roberto Pezza, Olga Mazina, Oleg Voloshin, R. Daniel Camerini-Otero, and Alexander Mazin
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External protocol30 Apr 2014

VINCULIN HEAD Domain 1 (Vd1 and Vd1 Y100E mutant): expression and purification

by Robert Liddington and Andrey Bobkov
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External protocol03 May 2014

TH17 cells contribute to uveitis and scleritis and are inhibited by IL-27/STAT1 in the retina. (4) Confocal microscopy

by Charles Egwuagu, Cheng-Rong Yu, Ahjoku Amadi-Obi, Xuebin Liu, Rashid Mahdi, and Yun Sang Lee
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External protocol30 Apr 2014

Aptamer-facilitated Protein Isolation from Cells

by Mirzo Kanoatov, Sahar Javaherian, and Sergey Krylov
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External protocol30 Apr 2014

Live-cell luciferase assay of drug resistant cells

by Kimi Honma and Takahiro Ochiya
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External protocol30 Apr 2014

Dendritic aggregation of the postsynaptic cell adhesion molecule NGL-3 by pre-clustered antibodies to induce postsynaptic protein clustering

by Jooyeon Woo, Seok-kyu Kwon, and Eunjoon Kim
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External protocol03 May 2014

Induction of IBD by transferring CD4+ CD45RB high cells in to SCID or Rag-/- mice

by Pushpa Pandiyan
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External protocol30 Apr 2014

Modeling evolution of insect resistance to genetically modified crops

by Bruce E. Tabashnik, David W. Crowder, and Yves Carrière
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External protocol30 Apr 2014

mRNA expression analysis by real-time PCR

by Ivan Zanoni and Renato Ostuni
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External protocol30 Apr 2014

A bioinformatics protocol for the identification of spatial clusters and the calculation of higher order residue interactions in protein structures

by Sundaramurthy Pandurangan, Shameer Khader, Raashi Sreenivasan, Sunita Gakkhar, and Sowdhamini Ramanathan
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