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Protocols

Protocols module of the Life Science Network enables sharing of detailed experimental protocols, laboratory recipes and data analysis procedures.

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External protocol03 May 2014

Rat Chromaffin cells primary cultures: Standardization and quality assessment for single-cell assays

by Juan A. Gilabert, Gema B. Montalvo, and Antonio R. Artalejo
- Views 263
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External protocol03 May 2014

Removal of CCR5 ligands and induction of pro-resolving lipid mediators by apoptotic neutrophils during resolution

by Charles N. Serhan, Amiram Ariel, Gabrielle Fredman, Yee-Ping Sun, Alpdogan Kantarci, Thomas E. Van Dyke, and Andrew D. Luster
- Views 289
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External protocol03 May 2014

Nucleofection and adoptive transfer of primary mouse T lymphocytes

by Lequn Li and Vassiliki Boussiotis
- Views 356
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External protocol03 May 2014

Combination of chemical cross-linking and pull-down assay to study transient protein-protein interactions

by Feng Gong, Deirdre Fahy, and Michael J. Smerdon
- Views 709
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External protocol03 May 2014

Determination of neopterin and biopterin by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) in rat and human plasma, cell extracts and tissue homogenates

by Helmut Schmidt, Irmgard Tegeder, and Gerd Geisslinger
- Views 583
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External protocol03 May 2014

Fluorescence in situ hybridization (FISH) for DNA replication origins

by Patrizia Gasparini and Omar Malazzi
- Views 650
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External protocol03 May 2014

Transient transfection and luciferase assay

by Karuppiah Muthumani
- Views 417
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External protocol03 May 2014

Cell-attached capacitance measurements at release sites of a central nerve terminal

by Liming He, Xin-Sheng Wu, Raja Mohan, and Ling-Gang Wu
- Views 267
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External protocol03 May 2014

Oscillating current stimulation – slow oscillation stimulation during sleep

by Lisa Marshall, Matthias Molle, and Jan Born
- Views 388
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External protocol03 May 2014

Sumoylation and desumoylation assays for a chromatin-remodelling complex in vivo and in vitro

by Jung Hwa Kim, Keun Il Kim, and Sung Hee Baek
- Views 426
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