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Protocols

Protocols module of the Life Science Network enables sharing of detailed experimental protocols, laboratory recipes and data analysis procedures.

You can upload a protocol here

External protocol30 Apr 2014

Isothermal titration calorimetry to determine the association constants for a ligand bound simultaneously to two specific protein binding sites with different affinities

by Giorgio Pochetti and Roberta Montanari
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External protocol30 Apr 2014

Ruthenium hydroxide supported on magnetic nanoparticles: a benign aqueous protocol for hydration of nitriles

by Vivek Polshettiwar and R. S. Varma
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External protocol30 Apr 2014

Improved genotyping of the dysferlin null mouse

by Renzhi Han, Kazuhiro Kobuke, Mary Anderson, Daniel Beltrán-Valero de Bernabé, Yvonne Kobayashi, Baoli Yang, and Kevin Campbell
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External protocol30 Apr 2014

Protocol for lentiviral knock down in mouse ES cells

by W.W.M. Pim Pijnappel, Marijke P.A. Baltissen, and H.T.Marc Timmers
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External protocol30 Apr 2014

VINCULIN TAIL (878-1065aa, Wt and Y1064E mutant), expression and purification

by Robert Liddington and Andrey Bobkov
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External protocol30 Apr 2014

Validation of artificial microRNA expression by poly(A) tailing-based RT-PCR

by Rui Shi, Chenmin Yang, Ronald Sederoff, and Vincent Chiang
- Views 243
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External protocol30 Apr 2014

Identification of protease cleavage-sites in E. coli lysate by differential N-terminomic analysis

by John C. Timmer and Guy S. Salvesen
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External protocol03 May 2014

Simultaneous inhibition and subsequent re-expression of multiple genes

by Xue-Ming Xu, Min-Hyuk Yoo, Bradley Carlson, and Dolph Hatfield
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External protocol30 Apr 2014

Radioisotopic Assay of L-carnitine (CN)

by Wei Zou
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External protocol30 Apr 2014

Optogenetic manipulation of Ventral Tegmental Area (VTA) Neurons that project to the Nucleus Accumbens (NAc) and medial Prefrontal Cortex (mPFC)

by Jessica Walsh*, Dipesh Chaudhury*, Allyson Friedman, Barbara Juarez, Stacy Ku, Mary Kay Lobo, and Ming-Hu Han
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