Latest Research Protocols - Life Science Network

External protocol03 May 2014

SLIC sub-cloning using T4 DNA polymerase treated inserts without RecA

by Mamie Z. Li and Stephen J. Elledge
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External protocol30 Apr 2014

Immunostaining of Nav1.6 and Nav1.2 channels in cortical pyramidal neurons

by Cuiping Tian, Wenqin Hu, and Yousheng Shu
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External protocol03 May 2014

TH17 cells contribute to uveitis and scleritis and are inhibited by IL-27/STAT1 in the retina. (3) Western Blot Analysis

by Charles Egwuagu, Cheng-Rong Yu, Ahjoku Amadi-Obi, Xuebin Liu, Rashid Mahdi, and Yun Sang Lee
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External protocol03 May 2014

Isolation of Treg cells and Treg cell suppression/death assay

by Pushpa Pandiyan
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External protocol10 Apr 2014

Mouse aortic ring assay: A new approach of the molecular genetics of angiogenesis

by Véronique Masson, Laetitia Devy, Christine Grignet-Debrus, Sarah Bernt, Khalid Bajou, Silvia Blacher, Guy Roland, Yawen Chang, Timothy Fong, Peter Carmeliet, Jean-Michel Foidart, Agnès Noël
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External protocol09 Apr 2014

Rapid cryopreservation of five mammalian and one mosquito cell line at −8°C while attached to flasks in a serum free cryopreservative

by Joe Corsini, Ethan Mann
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External protocol03 May 2014

Identifying novel p53-target genes and their functional analysis

by Wenwei Hu, Zhaohui Feng, Angelika Teresky, and Arnold Levine
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External protocol30 Apr 2014

Simultaneous semi-dry electrophoretic transfer of a wide range of differently sized proteins for immunoblotting

by Martin Wiedemann, Su Jung Lee, Richard Cardoso da Silva, Jyothsna Visweswaraiah, Josefin Soppert & Evelyn Sattlegger
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External protocol30 Apr 2014

Highly Sensitive Method for Titration of Adenovirus Vectors

by Hildegund Ertl, ZhiQuan Xiang, Yan Li, Dongming Zhou, Xiangyang Zhou, Wynetta Giles-Davis, and Yi-lin E. Liu
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External protocol09 Apr 2014

Comparative proteomics using 2-D gel electrophoresis and mass spectrometry as tools to dissect stimulons and regulons in bacteria with sequenced or partially sequenced genomes

by Sergio Encarnación, Magdalena Hernández, Gabriel Martínez-Batallar, Sandra Contreras, María del Vargas, Jaime Mora
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