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Protocols

Protocols module of the Life Science Network enables sharing of detailed experimental protocols, laboratory recipes and data analysis procedures.

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External protocol30 Apr 2014

Stripping protocol for Affymetrix tiling gene chips

by Hoi-Hung Cheung, Wai-Yee Chan, and Tin-Lap Lee
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External protocol30 Apr 2014

Subunit stoichiometry determination by perfluorooctanoic acid polyacrylamide gel electrophoresis (PFO-PAGE)

by Aubin Penna
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External protocol03 May 2014

SLIC sub-cloning using T4 DNA polymerase treated inserts without RecA

by Mamie Z. Li and Stephen J. Elledge
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External protocol03 May 2014

Directed yeast two hybrid screening for MEKK1 interaction partners

by Ewen Gallagher, Thomas Enzler, Atsushi Matsuzawa, and Michael Karin
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External protocol03 May 2014

Suppression of insulin target gene expression by SecinH3 quantified by real-time PCR

by Markus Hafner, Anton Schmitz, and Michael Famulok
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External protocol03 May 2014

Cell-attached capacitance measurements at release sites of a central nerve terminal

by Liming He, Xin-Sheng Wu, Raja Mohan, and Ling-Gang Wu
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External protocol03 May 2014

Rat Chromaffin cells primary cultures: Standardization and quality assessment for single-cell assays

by Juan A. Gilabert, Gema B. Montalvo, and Antonio R. Artalejo
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External protocol30 Apr 2014

Injection of Retrograde Beads into the Nucleus Accumbens (NAc) and Medial Prefronral Cortex (mPFC) to Isolate Projection-Specific Neurons in the Ventral Tegmental Area (VTA)

by Jessica Walsh*, Allyson Friedman*, Dipesh Chaudhury*, Barbara Juarez, Stacy Ku, and Ming-Hu Han
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External protocol30 Apr 2014

A method to target bioactive compounds to specific regions of the gastrointestinal tract: double gavage using polysaccharide hydrogels

by Hamed Laroui, Shanthi V. Sitaraman, and Didier Merlin
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External protocol30 Apr 2014

ACEMBLing multigene expression vectors by recombineering

by Yan Nie, Christoph Bieniossek, Daniel Frey, Natacha Olieric, Christiane Schaffitzel, Michel O. Steinmetz, and Imre Berger
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